MDH2 Antikörper (AA 9-223)

Produktnummer ABIN7602618

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch MDH2 in WB, IHC, ELISA, ICC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für MDH2 Antikörper (AA 9-223) (ABIN7602618)

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-MDH2 Antikörper

Bindungsspezifität: AA 9-223

Verwendungszweck: Anti-MDH2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-MDH2 Antibody Picoband® (ABIN7602618). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MDH2 recombinant protein (Position: A9-L223).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ait-El-Mkadem, S., Dayem-Quere, M., Gusic, M., Chaussenot, A., Bannwarth, S., Francois, B., Genin, E. C., Fragaki, K., Volker-Touw, C. L. M., Vasnier, C., Serre, V., van Gassen, K. L. I., and 24 others. Mutations in MDH2, encoding a Krebs cycle enzyme, cause early-onset severe encephalopathy. Am. J. Hum. Genet. 100: 151-159, 2017. 2. Benn, P., Chern, C. J., Bruns, G., Craig, I. W., Croce, C. M. Assignment of the genes for human beta-glucuronidase and mitochondrial malate dehydrogenase to the region pter-q22 of chromosome 7. Cytogenet. Cell Genet. 19: 273-280, 1977. 3. Blake, N. M. Malate dehydrogenase types in the Asian-Pacific area, and a description of new phenotypes. Hum. Genet. 43: 69-80, 1978.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu MDH2

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Andere Bezeichnung: MDH2

Hintergrund:

Synonyms: Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150 kDa component, ARC150, Cofactor required for Sp1 transcriptional activation subunit 2, CRSP complex subunit 2, Mediator complex subunit 14, RGR1 homolog, hRGR1, Thyroid hormone receptor-associated protein complex 170 kDa component, Trap170, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150 kDa component, DRIP150, MED14, ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170,

Tissue Specificity: Ubiquitous.

Background: Malate dehydrogenase, mitochondrial also known as malate dehydrogenase 2 is an enzyme that in humans is encoded by the MDH2 gene. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 36 kDa

Gen-ID: 4191

UniProt: P40926